Name and publication

Phytophthora amnicola T.I. Burgess & T. Jung (2012)

Burgess TI, Hüberli D, Hardy GE StJ, Stukely MJC, and Jung T. 2012. Phytophthora amnicola T. I. Burgess & T. Jung, sp. nov. Persoonia 28: 140–141.

Corresponding author tburgess@murdoch.edu.au

Nomenclature

from Burgess et al. (2012)

Mycobank

MB563849

Etymology

named for the riverside habitat of this species

Typification

Type: WESTERN AUSTRALIA, Perth, Poison Gully Creek, baited from still water, Dec. 2009, D. Hüberli, holotype MURU 471

Ex-type: cultures ex-type CBS 131652 = DH228

Sequences for Ex-type in original manuscript

CBS 131652 = DH228 = ITS JQ029956, β-tubulin JQ029952, HSP90 JQ029944, cox1 JQ029948, NADH JQ029940, and LSU JX069838

Ex-type in other collections

CBS 131652 = DH228 = P19862 (WPC), CPHST-BL 100 (Abad), 61G6 (Yang)

Ex-type in other collections

(ET) CBS 131652, DH228, WPC P19862, S&T BL 100 (Abad), 61G6 (Hong)    

Molecular identification

Voucher sequences for barcoding genes (ITS rDNA and COI) of the ex-type (see Molecular protocols page)

Phytophthora amnicola isolate DH228 ITS rDNA JQ029956

Phytophthora amnicola isolate CPHST BL 100 (= P19862 WPC) COI MH477740

Voucher sequences for Molecular Toolbox with seven genes (ITS, β-tub, COI, EF1α, HSP90, L10, and YPT1 

(see Molecular protocols page) (In Progress)

Voucher sequences for Metabarcoding High-throughput Sequencing (HTS) Technologies [Molecular Operational Taxonomic Unit (MOTU)]

(see Molecular protocols page) (In Progress)

Sequences with multiple genes for ex-type in other sources

• NCBI: Phytophthora amnicola CPHST BL 100
• NCBI: Phytophthora amnicola DH228
• BOLDSYSTEMS: Phytophthora amnicola (barcoding COI & ITS)

Position in multigenic phylogeny with 7 genes (ITS, β-tub, COI, EF1α, HSP90, L10, and YPT1)

Clade clade:
a taxonomic group of organisms classified together on the basis of homologous features traced to a common ancestor
6b

Morphological identification

Colonies and cardinal temperatures

Colony colony:
assemblage of hyphae which usually develops form a single source and grows in a coordinated way
morphology is stellate with limited aerial myceliummycelium:
mass of hyphae constituting the body, or thallus, of fungi and oomycetes; in <em>Phytophthora</em> species, mycelia is coenocytic
on V8 agar, rosaceous on carrot agar, and growth on potato-dextrose agar is very slow. Minimum growth temperature 7.5°C, optimum 25–32.5°C, and maximum 37.5°C.

Conditions for growth and sporulation

SporangiaSporangia:
sac within which zoospores form, especially when water is cooled to about 10°C below ambient temperature; in solid substrates, sporangia usually germinate by germ tubes
 are produced in water cultures (soil extract or river water) and not observed in solid media. OogoniaOogonia:
the female gametangium in which the oospore forms after fertilization by the antheridium
are not observed in single culture or when paired with tester strains.

Asexual phase

SporangiaSporangia:
sac within which zoospores form, especially when water is cooled to about 10°C below ambient temperature; in solid substrates, sporangia usually germinate by germ tubes
 are nonpapillatenonpapillate:
pertaining to the production of a non-distinct, or inconspicuous, papilla at the distal end of the sporangium (cf. papillate and semipapillate)
, persistentpersistent:
pertaining to sporangia that remain attached to the sporangiophore and do not separate or detach easily (cf. caducous)
, ovoidovoid:
egg-shaped, with the widest part at the base of the sporangium and the narrow part at the apex
, or limoniform with average dimensions of 62 ± 9.0 × 35.3 ± 5.6 μm (overall range 39–78 × 17–43 μm). Sporangiophores in simple sympodiasympodia:
a type of sporangiophore which appears simple, but where each successive sporangium develops on a branch behind and to one side of the previous apex, where growth has already ceased
and internal proliferationinternal proliferation:
internal proliferation occurs when the sporangiophore continues to grow through an empty sporangium
, both nested and extended, occur in chains. Hyphal swellings are ellipsoidellipsoid:
refers to a solid body that forms an ellipse in the longitudinal plane and a circle in cross section; many fungal spores are ellipsoidal or elliptic
to irregular, catenulatecatenulate:
having a chain-like form
occurring in clusters. ChlamydosporesChlamydospores:
an asexual spore with a thickened inner wall&nbsp;that is delimited from the mycelium by a septum; may be terminal or intercalary, and survives for long periods in soil
 absent

Sexual phase

Sterile in culture.

Most typical characters

Phytophthora amnicola is in a species cluster with Phytophthora litoralis, Phytophthora fluvialis, Phytophthora moyootj, and Phytophthora thermophila, all recovered from waterways in Western Australia. There are few features to distinguish these species, however P. amnicola is the only species that produces rosaceous colonies on CA.

Specimen(s) evaluated

Australia, Western Australia, Perth, Poison Gully Creek, baited from still water, Dec. 2009, D. Hüberli, CBS131652 = DH228; Lake Jualbup, DH013; Canning River, DH237; Pemberton, 2008, VHS19503

CPHST-BL 100 (ET) = P19862 World Phytophthora Collection – California, USA

Hosts and distribution

Distribution: Western Australia
Substrate: water
Disease note: No pathogenicity trials have been conducted. The species has been recovered from dying blackberry corms, but pathogenicity is not known.
Hosts: known only from water

Retrieved January 18, 2018 from U.S. National Fungus Collections Nomenclature Database.

Additional references and links

Crous PW, Summerell BA, Shivas RG, Burgess TI, Decock CA, Dreyer LL, Granke LL, Guest DI, Hardy GESJ, Hausbeck MK, Hüberli D, Jung T, Koukol O, Lennox CL, Liew ECY, Lombard L, McTaggart AR, Pryke JS, Roets F, Saude C, Shuttleworth LA, Stukely MJC, Vánky K, Webster BJ, Windstam ST, and Groenewald JZ. 2012. Fungal Planet description sheets: 107–127. Persoonia 28: 138–182.

Jung T, Stukely MJC, Hardy GEStJ, White D, Paap T, and Burgess TI. 2011. Multiple new Phytophthora species from ITS Cladeclade:
a taxonomic group of organisms classified together on the basis of homologous features traced to a common ancestor
6 associated with natural ecosystems in Australia: evolutionary and ecological implications. Persoonia 26: 13–39.

Hüberli D, Hardy GESJ, White D, Williams N, and Burgess TI. 2013. Fishing for Phytophthora from Western Australia's waterways: A distribution and diversity survey. Australasian Plant Pathology 42: 251–260

• SMML USDA-ARS: Phytophthora amnicola
• EPPO Global Database: Phytophthora amnicola
• Forest Phytophthoras of the World: Phytophthora amnicola
• CABI Digital Library: Phytophthora amnicola
• Encyclopedia of Life (EOL): Phytophthora amnicola
• Index Fungorum (IF): Phytophthora amnicola

 

• Google All Phytophthora amnicola
• Google Images Phytophthora amnicola
• Google Scholar Phytophthora amnicola

 

Fact sheet authors

Treena Burgess, Ph.D., Phytophthora Science and Management, Harry Butler Institute, Murdoch University, Australia

Z. Gloria Abad, Ph.D., USDA-APHIS-PPQ-S&T Plant Pathogen Confirmatory Diagnostics Laboratory (PPCDL), United States of America.