Name and publication

Phytophthora celebensis Jung, M. Junaid, N. Nasri & I. Milenković (2022)

Jung T, Milenković I, Corcobado T. et al. 2022. Extensive morphological and behavioural diversity among fourteen new and seven described species in Phytophthora Clade clade:
a taxonomic group of organisms classified together on the basis of homologous features traced to a common ancestor
10 and its evolutionary implications. Persoonia-Molecular Phylogeny and Evolution of Fungi 49: 1–57.

Corresponding authors: thomas.jung@mendelu.cz and dr.t.jung@gmail.com

Nomenclature

Mycobank

MB843002

Etymology

Name refers to the origin of all known isolates in Sulawesi (Celebes is a previous name of Sulawesi).

Typification

Type: Indonesia, South Sulawesi Province, District of Tana Toraja, isolated from a naturally fallen leaf floating in a stream running through a submontane tropical rainforest, May 2019, T. Jung, M. Junaid & N. Nasri, holotype HNHM-MYC-021540, dried culture on V8A, Herbarium of the Hungarian Natural History Museum, Budapest

Ex-type: culture CBS 148800 = SL092

Sequences for ex-type in original manuscript:  LSU ON000626, ITS ON000720, β-tubulin OM975899, hsp90 OM976416, tigA OM974594, rpl10 OM974453, tef-1a OM984880, enolase OM976512, cox1 ON013786, ypt1 ON024938, nadh1 OM976896, rps10 OM976654

Ex-type in other collections

(ET) CBS 148800, SL092 

Molecular identification

Voucher sequences for barcoding genes (ITS rDNA and COI) of the ex-type (see Molecular protocols page)

Phytophthora celebensis ITS rDNA, COI

Voucher sequences for Molecular Toolbox with seven genes (ITS, β-tub, COI, EF1α, HSP90, L10, and YPT1

(see Molecular protocols page) (In Progress)

Voucher sequences for Metabarcoding High-throughput Sequencing (HTS) Technologies [Molecular Operational Taxonomic Unit (MOTU)]

(see Molecular protocols page) (In Progress)

Sequences with multiple genes for ex-type in other sources

• NCBI: Phytophthora celebensis
• EPPO-Q-bank: Phytophthora celebensis
• BOLDSYSTEMS: Phytophthora celebensis

Position in multigenic phylogeny with 7 genes (ITS, β-tub, COI, EF1α, HSP90, L10, and YPT1)

Clade clade:
a taxonomic group of organisms classified together on the basis of homologous features traced to a common ancestor
10a

Morphological identification

Colonies and cardinal temperatures

Colony colony:
assemblage of hyphae which usually develops form a single source and grows in a coordinated way
morphology appressed with a dendroid growth pattern on V8A and CA, and dense-woolly and uniform on PDA. Optimum growth temperature 25°C and maximum 27.5°C.

Conditions for growth and sporulation

SporangiaSporangia:
sac within which zoospores form, especially when water is cooled to about 10°C below ambient temperature; in solid substrates, sporangia usually germinate by germ tubes
produced in non-sterile soil extract after 1–2d. OogoniaOogonia:
the female gametangium in which the oospore forms after fertilization by the antheridium
produced readily in single culture on V8A after 2–3 weeks.

Asexual phase

SporangiaSporangia:
sac within which zoospores form, especially when water is cooled to about 10°C below ambient temperature; in solid substrates, sporangia usually germinate by germ tubes
were papillatepapillate:
pertaining to the production of a distinct papilla at the distal end of the sporangium (cf. nonpapillate and semipapillate)
, caducouscaducous:
pertaining to sporangia that become dislodged readily (i.e. deciduous) and separate from the sporangiophore (cf. persistent)
, and predominantly ovoidovoid:
egg-shaped, with the widest part at the base of the sporangium and the narrow part at the apex
to broad ovoidovoid:
egg-shaped, with the widest part at the base of the sporangium and the narrow part at the apex
and limoniform in shape. SporangiaSporangia:
sac within which zoospores form, especially when water is cooled to about 10°C below ambient temperature; in solid substrates, sporangia usually germinate by germ tubes
averaged 38.7 x 26.2 µm (overall range 26.3–64.3 x 17.6–40.7 µm), external proliferationexternal proliferation:
formation of a sporangium after a sporangiophore has emerged from beneath and external to an empty sporangium that has previously emitted its zoospores (cf. internal proliferation)
. Sporangiophores dense sympodiasympodia:
a type of sporangiophore which appears simple, but where each successive sporangium develops on a branch behind and to one side of the previous apex, where growth has already ceased
. Hyphal swellings absent. ChlamydosporesChlamydospores:
an asexual spore with a thickened inner wall that is delimited from the mycelium by a septum; may be terminal or intercalary, and survives for long periods in soil
absent.

Sexual phase

Homothallic. OogoniaOogonia:
the female gametangium in which the oospore forms after fertilization by the antheridium
smooth-walled, 16–43 µm;. OosporesOospores:
zygote or thick-walled spore that forms within the oogonium after fertilization by the antheridium; may be long-lived
predominantly pleroticplerotic:
pertaining to an oospore that fills the oogonium (cf. aplerotic)
, size ranged 14–39 µm. AntheridiaAntheridia:
the male gametangium; a multinucleate, swollen hyphal tip affixed firmly to the wall of the female gametangium (the oogonium)
amphigynousamphigynous:
pertaining to the sexual stage in which the antheridium completely surrounds the stalk of the oogonium (cf. paragynous)
.

Most typical characters

Resides in phylogenetic cladeclade:
a taxonomic group of organisms classified together on the basis of homologous features traced to a common ancestor
10a and is closely related to P. multiglobulosa and P. javanensis. These species have similar morphology and have to date been recovered only from Indonesia.

Additional specimen(s) evaluated

Indonesia, South Sulawesi Province, District of Tana Toraja, isolated from naturally fallen leaves floating in a stream running through a submontane tropical rainforest, May 2019, T. Jung, M. Junaid & N. Nasri, SL091, SL540, SL541, SL542.

Hosts and distribution

Distribution: Indonesia
Substrate: fallen leaves in forest stream
Disease note: no known disease
Host: no known host

Additional references and links

• SMML USDA-ARS: Phytophthora celebensis
• EPPO Global Database: Phytophthora celebensis
• Forest Phytophthoras of the World: Phytophthora celebensis
• CABI Digital Library: Phytophthora celebensis
• Encyclopedia of Life (EOL): Phytophthora celebensis
• Index Fungorum (IF): Phytophthora celebensis

Fact sheet author

Treena Burgess, Ph.D., Phytophthora Science and Management, Harry Butler Institute, Murdoch University, Australia

Z. Gloria Abad, Ph.D., USDA-APHIS-PPQ-S&T Plant Pathogen Confirmatory Diagnostics Laboratory (PPCDL), United States of America.