Name and publication

Phytophthora multiglobulosa Jung, M. Junaid, M. Horta, Jung & I. Milenković (2022)

Jung T, Milenković I, Corcobado T. et al. 2022. Extensive morphological and behavioural diversity among fourteen new and seven described species in Phytophthora Clade clade:
a taxonomic group of organisms classified together on the basis of homologous features traced to a common ancestor
10 and its evolutionary implications. Persoonia-Molecular Phylogeny and Evolution of Fungi 49: 1–57.

Corresponding authors: thomas.jung@mendelu.cz and dr.t.jung@gmail.com

Nomenclature

Mycobank

MB843003

Etymology

Name refers to the presence of multiple lipid globules in many oosporesoospores:
zygote or thick-walled spore that forms within the oogonium after fertilization by the antheridium; may be long-lived
.

Typification

Type: Indonesia, South Sulawesi Province, District of North Toraja, isolated from a naturally fallen leaf floating in a stream running through a tropical hill rainforest, May 2019, T. Jung, M. Junaid & N. Nasri, holotype HNHMMYC- 021539,, dried culture on V8A, Herbarium of the Hungarian Natural History Museum, Budapest

Ex-type: culture CBS 148799 = SL005

Sequences for ex-type in original manuscript:  LSU ON000669, ITS ON000763, β-tubulin OM975942, hsp90 OM976459, tigA OM974636, rpl10 OM974496, tef-1a OM984922, enolase OM976554, cox1 ON013829, ypt1 ON024979, nadh1 OM976939, rps10 OM976697

Ex-type in other collections

(ET) CBS 148799, SL005 

Molecular identification

Voucher sequences for barcoding genes (ITS rDNA and COI) of the ex-type (see Molecular protocols page)

Phytophthora multiglobulosa ITS rDNA, COI

Voucher sequences for Molecular Toolbox with seven genes (ITS, β-tub, COI, EF1α, HSP90, L10, and YPT1

(see Molecular protocols page) (In Progress)

Voucher sequences for Metabarcoding High-throughput Sequencing (HTS) Technologies [Molecular Operational Taxonomic Unit (MOTU)]

(see Molecular protocols page) (In Progress)

Sequences with multiple genes for ex-type in other sources

• NCBI: Phytophthora multiglobulosa
• EPPO-Q-bank: Phytophthora multiglobulosa
• BOLDSYSTEMS: Phytophthora multiglobulosa

Position in multigenic phylogeny with 7 genes (ITS, β-tub, COI, EF1α, HSP90, L10, and YPT1)

Clade clade:
a taxonomic group of organisms classified together on the basis of homologous features traced to a common ancestor
10a

Morphological identification

Colonies and cardinal temperatures

Colony colony:
assemblage of hyphae which usually develops form a single source and grows in a coordinated way
morphology submerged to appressed colonies with a radiate pattern and irregular margins on V8A and CA, and dense-woolly and uniform on PDA. Optimum growth temperature 20°C and maximum 27.5°C.

Conditions for growth and sporulation

SporangiaSporangia:
sac within which zoospores form, especially when water is cooled to about 10°C below ambient temperature; in solid substrates, sporangia usually germinate by germ tubes
produced in non-sterile soil extract after 1–2d. OogoniaOogonia:
the female gametangium in which the oospore forms after fertilization by the antheridium
produced readily in single culture on V8A after 2–3 weeks.

Asexual phase

SporangiaSporangia:
sac within which zoospores form, especially when water is cooled to about 10°C below ambient temperature; in solid substrates, sporangia usually germinate by germ tubes
were papillatepapillate:
pertaining to the production of a distinct papilla at the distal end of the sporangium (cf. nonpapillate and semipapillate)
, caducouscaducous:
pertaining to sporangia that become dislodged readily (i.e. deciduous) and separate from the sporangiophore (cf. persistent)
, and predominantly ovoidovoid:
egg-shaped, with the widest part at the base of the sporangium and the narrow part at the apex
and limoniform in shape. SporangiaSporangia:
sac within which zoospores form, especially when water is cooled to about 10°C below ambient temperature; in solid substrates, sporangia usually germinate by germ tubes
averaged 43.1 x 27.1 µm (overall range 28.3–64.9 x 21.1–36.5 µm), external proliferationexternal proliferation:
formation of a sporangium after a sporangiophore has emerged from beneath and external to an empty sporangium that has previously emitted its zoospores (cf. internal proliferation)
. Sporangiophores dense sympodiasympodia:
a type of sporangiophore which appears simple, but where each successive sporangium develops on a branch behind and to one side of the previous apex, where growth has already ceased
. Hyphal swellings absent. ChlamydosporesChlamydospores:
an asexual spore with a thickened inner wall that is delimited from the mycelium by a septum; may be terminal or intercalary, and survives for long periods in soil
absent.

Sexual phase

Homothallic. OogoniaOogonia:
the female gametangium in which the oospore forms after fertilization by the antheridium
smooth-walled, 17–38 µm. OosporesOospores:
zygote or thick-walled spore that forms within the oogonium after fertilization by the antheridium; may be long-lived
pleroticplerotic:
pertaining to an oospore that fills the oogonium (cf. aplerotic)
containing multiple lipid globules, size ranged 15–35 µm. AntheridiaAntheridia:
the male gametangium; a multinucleate, swollen hyphal tip affixed firmly to the wall of the female gametangium (the oogonium)
amphigynousamphigynous:
pertaining to the sexual stage in which the antheridium completely surrounds the stalk of the oogonium (cf. paragynous)
.

Most typical characters

Resides in phylogenetic cladeclade:
a taxonomic group of organisms classified together on the basis of homologous features traced to a common ancestor
10a and is closely related to P. celebensis and P. javanensis. These species have similar morphology and have to date been recovered only from tropical rainforests streams in Indonesia.

Additional specimen(s) evaluated

Indonesia, South Sulawesi Province, District of North Toraja, isolated from naturally fallen leaves floating in a stream running through a tropical hill rainforest, May 2019, T. Jung, M. Junaid & N. Nasri, SL006, SL007

Hosts and distribution

Distribution: Indonesia
Substrate: fallen leaves in forest stream
Disease note: no known disease
Host: no known host

Additional references and links

• SMML USDA-ARS: Phytophthora multiglobulosa
• EPPO Global Database: Phytophthora multiglobulosa
• Forest Phytophthoras of the world: Phytophthora multiglobulosa
• CABI Digital Library: Phytophthora multiglobulosa
• Encyclopedia of Life (EOL): Phytophthora multiglobulosa
• Index Fungorum (IF): Phytophthora multiglobulosa

Fact sheet author

Treena Burgess, Ph.D., Phytophthora Science and Management, Harry Butler Institute, Murdoch University, Australia

Z. Gloria Abad, Ph.D., USDA-APHIS-PPQ-S&T Plant Pathogen Confirmatory Diagnostics Laboratory (PPCDL), United States of America.