Name and publication

Phytophthora oleae Schena, Ruano-Rosa, Agosteo & Cacciola (2018)

Ruano-Rosa D, Schena L, Agosteo GE, Magnano di San Lio G, and Cacciola SO. 2018. Phytophthora oleae sp. nov. causing fruit rot of olive in southern Italy. Plant Pathology 67: 1362–1373.

Corresponding author: olgacacciola@unict.it

Nomenclature

from Ruano-Rosa et al. (2018)

Mycobank

MB824218

Etymology

refers to the matrix (fruits of Olea europaea) from which this species was recovered

Typification

Type: ITALY, Calabria region, Vibo Valentia province, isolated from rotten drupes of olive (Olea europaea, Oleaceae family) in a commercial olive orchard at Mileto (Vibo Valentia province), in the middle of November 2015, collectors G.E. Agosteo and L. Schena; isolate Po1a; holotype: RCPH3, dried culture on V8A in the herbarium of the Department of Agriculture at the University Mediterranea of Reggio Calabria, Reggio Calabria, Italy

Ex-type: CBS 7669 (preserved as living culture at CBS-KNAW Fungal 307 Biodiversity Centre, Utrecht, The Netherlands)

Sequences for ex-type in original manuscript:

Phytophthora oleae isolate Po1a ITS KY982930

Ex-type in other collections

(ET) CBS 141871, Po1a, NRRL 64313, S&T BL 219 (Abad)

Molecular identification

Voucher sequences for barcoding genes (ITS rDNA and COI) of the ex-type (see Molecular protocols page)

Phytophthora oleae Po1a = ITS rDNA KY982930, COI MF083569

Voucher sequences for Molecular Toolbox with seven genes (ITS, β-tub, COI, EF1α, HSP90, L10, and YPT1

(see Molecular protocols page) (In Progress)

Voucher sequences for Metabarcoding High-throughput Sequencing (HTS) Technologies [Molecular Operational Taxonomic Unit (MOTU)]

(see Molecular protocols page) (In Progress)

Sequences with multiple genes for ex-type in other sources

• NCBI: Phytophthora oleae Po1a
• EPPO-Q-bank: Phytophthora oleae
• BOLDSYSTEMS: Phytophthora oleae (barcoding COI & ITS)

Position in multigenic phylogeny with 7 genes (ITS, β-tub, COI, EF1α, HSP90, L10, and YPT1)

Clade clade:
a taxonomic group of organisms classified together on the basis of homologous features traced to a common ancestor
2d

Morphological identification

adapted from Ruano-Rosa et al. (2018)

Colonies and cardinal temperatures

Colonies with a stellate to radial growth pattern on PDA, rosaceous on CA, and abundant aerial myceliummycelium:
mass of hyphae constituting the body, or thallus, of fungi and oomycetes; in <em>Phytophthora</em> species, mycelia is coenocytic
with no definite pattern on V8A. Cardinal temperatures (minimum, maximum, and optimum) for radial growth 10ºC, 25ºC, and 20ºC, respectively.

Conditions for growth and sporulation

Sexual reproductive structures were produced in single culture on V8A after one week. SporangiaSporangia:
sac within which zoospores form, especially when water is cooled to about 10°C below ambient temperature; in solid substrates, sporangia usually germinate by germ tubes
abundant in water culture, less numerous on solid agar.

Asexual phase

SporangiaSporangia:
sac within which zoospores form, especially when water is cooled to about 10°C below ambient temperature; in solid substrates, sporangia usually germinate by germ tubes
semipapillatesemipapillate:
pertaining to the production of shallow having papilla that are not well developed, shallow and less nipple-like than fully papillate structures
, occasionally bipapillate; persistentpersistent:
pertaining to sporangia that remain attached to the sporangiophore and do not separate or detach easily (cf. caducous)
; ovoidovoid:
egg-shaped, with the widest part at the base of the sporangium and the narrow part at the apex
(32–58 µm length x 22–36 µm width), originated in lax, simple sympodial sporangiophoresporangiophore:
the hyphal strand on which the sporangium is formed; may be branched or unbranched to form compound sympodia or simple sympodia
, sometimes sessile. Hyphal swellings catenulatecatenulate:
having a chain-like form
and simple; intercalary are sporadically observed. ChlamydosporesChlamydospores:
an asexual spore with a thickened inner wall&nbsp;that is delimited from the mycelium by a septum; may be terminal or intercalary, and survives for long periods in soil
are also very sporadic and produced only on solid media.

Sexual phase

Homothallic. Oogonia smooth-walled, globoseglobose:
having a rounded form resembling that of a sphere
to subglobose (24–30 μm diam.); antheridia paragynous, club-shaped, and rarely with a finger-like appendix; oosporesoospores:
zygote or thick-walled spore that forms within the oogonium after fertilization by the antheridium; may be long-lived
pleroticplerotic:
pertaining to an oospore that fills the oogonium (cf. aplerotic)
(22–28 μm diam.).

Additional references and links

 

• SMML USDA-ARS: Phytophthora oleae
• EPPO Global Database: Phytophthora oleae
• Forest Phytophthoras of the World: Phytophthora oleae
• CABI Digital Library: Phytophthora oleae
• Encyclopedia of Life (EOL): Phytophthora oleae
• Index Fungorum (IF): Phytophthora oleae

 

 

• Google All Phytophthora oleae
• Google Images Phytophthora oleae
• Google Scholar Phytophthora oleae

 

Fact sheet author

Z. Gloria Abad, Ph.D., USDA-APHIS-PPQ-S&T Plant Pathogen Confirmatory Diagnostics Laboratory (PPCDL), United States of America.