Name and publication

Phytophthora pseudogallica T. Jung, N.M. Chi, Brasier & I. Milenković (2022)

Jung T, Milenković I, Corcobado T. et al. 2022. Extensive morphological and behavioural diversity among fourteen new and seven described species in Phytophthora Cladeclade:
a taxonomic group of organisms classified together on the basis of homologous features traced to a common ancestor
10 and its evolutionary implications. Persoonia-Molecular Phylogeny and Evolution of Fungi 49: 1–57.

Corresponding authors: thomas.jung@mendelu.cz and dr.t.jung@gmail.com

Nomenclature

Mycobank

MB 842950

Etymology

Name refers to the morphological similarity to P. gallica.

Typification

Type: Sapa, isolated from a fallen leaf in a stream running through an evergreen cloud forest, 2017, T. Jung & N.M. Chi, holotype HNHM-MYC-009706, dried culture on V8A, Herbarium of the Hungarian Natural History Museum, Budapest, Hungary

Ex-type: culture CBS 149206 = NRRL 64136 = VN861

Sequences for ex-type in original manuscript:  LSU ON000680, ITS ON000774, β-tubulin OM975953, hsp90 OM976470, tigA OM974647, rpl10 OM97507, tef-1a OM984933, enolase OM976564, cox1 ON013840, ypt1 ON024990, nadh1 OM976949, rps10 OM976708

Ex-type in other collections

(ET) CBS 149206, NRRL 64136, VN861 

Molecular identification

Voucher sequences for barcoding genes (ITS rDNA and COI) of the ex-type (see Molecular protocols page)

Phytophthora pseudogallica ITS rDNA, COI

Voucher sequences for Molecular Toolbox with seven genes (ITS, β-tub, COI, EF1α, HSP90, L10, and YPT1

(see Molecular protocols page) (In Progress)

Voucher sequences for Metabarcoding High-throughput Sequencing (HTS) Technologies [Molecular Operational Taxonomic Unit (MOTU)]

(see Molecular protocols page) (In Progress)

Sequences with multiple genes for ex-type in other sources

• NCBI: Phytophthora pseudogallica
• EPPO-Q-bank: Phytophthora pseudogallica
• BOLDSYSTEMS: Phytophthora pseudogallica

Position in multigenic phylogeny with 7 genes (ITS, β-tub, COI, EF1α, HSP90, L10, and YPT1)

Clade clade:
a taxonomic group of organisms classified together on the basis of homologous features traced to a common ancestor
10b

Morphological identification

Colonies and cardinal temperatures

Colony colony:
assemblage of hyphae which usually develops form a single source and grows in a coordinated way
morphology striate on V8A with white aerial myceliummycelium:
mass of hyphae constituting the body, or thallus, of fungi and oomycetes; in <em>Phytophthora</em> species, mycelia is coenocytic
in the center and appressed to submerged margins, uniform and dense-felty on CA, and stoloniferous dense-felty on PDA. Optimum growth temperature 20°C and maximum 25°C.

Conditions for growth and sporulation

SporangiaSporangia:
sac within which zoospores form, especially when water is cooled to about 10°C below ambient temperature; in solid substrates, sporangia usually germinate by germ tubes
produced in non-sterile soil extract after 2–3d. OogoniaOogonia:
the female gametangium in which the oospore forms after fertilization by the antheridium
were not produced in paired cultures using several known tester strains.

Asexual phase

SporangiaSporangia:
sac within which zoospores form, especially when water is cooled to about 10°C below ambient temperature; in solid substrates, sporangia usually germinate by germ tubes
were nonpapillatenonpapillate:
pertaining to the production of a non-distinct, or inconspicuous, papilla at the distal end of the sporangium (cf. papillate and semipapillate)
, persistentpersistent:
pertaining to sporangia that remain attached to the sporangiophore and do not separate or detach easily (cf. caducous)
, and predominantly ovoidovoid:
egg-shaped, with the widest part at the base of the sporangium and the narrow part at the apex
to broad ovoidovoid:
egg-shaped, with the widest part at the base of the sporangium and the narrow part at the apex
in shape. SporangiaSporangia:
sac within which zoospores form, especially when water is cooled to about 10°C below ambient temperature; in solid substrates, sporangia usually germinate by germ tubes
averaged 25.2 x 21.3 µm (overall range 19.4–33.7 x 13.0–29.5 µm), proliferationproliferation:
formation of a sporangium within an empty sporangium that has previously emitted zoospores (internal or nested) or after the sporangiophore has emerged from the empty sporangium (external)
internal, nested, and extended. Sporangiophores unbranched. Hyphal swellings absent. ChlamydosporesChlamydospores:
an asexual spore with a thickened inner wall&nbsp;that is delimited from the mycelium by a septum; may be terminal or intercalary, and survives for long periods in soil
present, golden brown.

Sexual phase

Sterile in culture.

Most typical characters

Resides in phylogenetic cladeclade:
a taxonomic group of organisms classified together on the basis of homologous features traced to a common ancestor
10b, and is most closely related to P. tonkinensis, but differs by not producing oosporesoospores:
zygote or thick-walled spore that forms within the oogonium after fertilization by the antheridium; may be long-lived
in culture. Morphologically it resembles P. gallica, another species from cladeclade:
a taxonomic group of organisms classified together on the basis of homologous features traced to a common ancestor
10b.

Additional specimen(s) evaluated

Vietnam, Sapa, isolated from fallen leaves in a stream running through an evergreen cloud forest, 2017, T. Jung & N.M. Chi, VN910, VN920, VN922.

Hosts and distribution

Distribution: Vietnam
Substrate: fallen leaf in stream
Disease note: no known disease
Host: no known host

Additional references and links

• SMML USDA-ARS: Phytophthora pseudogallica
• EPPO Global Database: Phytophthora pseudogallica
• Forest Phytophthoras of the world: Phytophthora pseudogallica
• CABI Digital Library: Phytophthora pseudogallica
• Encyclopedia of Life (EOL): Phytophthora pseudogallica
• Index Fungorum (IF): Phytophthora pseudogallica

Fact sheet author

Treena Burgess, Ph.D., Phytophthora Science and Management, Harry Butler Institute, Murdoch University, Australia

Z. Gloria Abad, Ph.D., USDA-APHIS-PPQ-S&T Plant Pathogen Confirmatory Diagnostics Laboratory (PPCDL), United States of America.