Name and publication

Phytophthora ukrainensis I. Milenković, T. Jung, T. Corcobado & I. Matsiakh (2022)

Jung T, Milenković I, Corcobado T. et al. 2022. Extensive morphological and behavioural diversity among fourteen new and seven described species in Phytophthora Clade clade:
a taxonomic group of organisms classified together on the basis of homologous features traced to a common ancestor
10 and its evolutionary implications. Persoonia-Molecular Phylogeny and Evolution of Fungi 49: 1–57.

Corresponding authors: thomas.jung@mendelu.cz and dr.t.jung@gmail.com

Nomenclature

Mycobank

MB 843136

Etymology

Name refers to the origin of most known isolates in Ukraine.

Typification

Type: Ukraine, Lviv region, Ivano-Frankove, isolated from a naturally fallen Quercus leaf in the Vereshchytsia River, Aug. 2019, I. Milenković, T. Corcobado & I. Matsiakh, holotype HNHM-MYC-009710, dried culture on V8A, Herbarium of the Hungarian Natural History Museum, Budapest

Ex-type: culture CBS 148851 = NRRL 64255 = UA373

Sequences for ex-type in original manuscript:  LSU ON000711, ITS ON000805, β-tubulin OM975984, hsp90 OM976501, tigA OM974679, rpl10 OM97539, enolase OM976596, cox1 ON013872, ypt1 ON025022, nadh1 OM976981, rps10 OM976740

Ex-type in other collections

(ET) CBS 148851, NRRL 64255, UA373 

Molecular identification

Voucher sequences for barcoding genes (ITS rDNA and COI) of the ex-type (see Molecular protocols page)

Phytophthora ukrainensis ITS rDNA, COI

Voucher sequences for Molecular Toolbox with seven genes (ITS, β-tub, COI, EF1α, HSP90, L10, and YPT1

(see Molecular protocols page) (In Progress)

Voucher sequences for Metabarcoding High-throughput Sequencing (HTS) Technologies [Molecular Operational Taxonomic Unit (MOTU)]

(see Molecular protocols page) (In Progress)

Sequences with multiple genes for ex-type in other sources

• NCBI: Phytophthora ukrainensis
• EPPO-Q-bank: Phytophthora ukrainensis
• BOLDSYSTEMS: Phytophthora ukrainensis

Position in multigenic phylogeny with 7 genes (ITS, β-tub, COI, EF1α, HSP90, L10, and YPT1)

Clade clade:
a taxonomic group of organisms classified together on the basis of homologous features traced to a common ancestor
10c

Morphological identification

Colonies and cardinal temperatures

Colony colony:
assemblage of hyphae which usually develops form a single source and grows in a coordinated way
morphology submerged and radiate on CA, submerged and striate on V8A, and stoloniferous and dense felty on PDA. Optimum growth temperature 20°C and maximum 32.5°C.

Conditions for growth and sporulation

SporangiaSporangia:
sac within which zoospores form, especially when water is cooled to about 10°C below ambient temperature; in solid substrates, sporangia usually germinate by germ tubes
produced in non-sterile soil extract after 1–2d. OogoniaOogonia:
the female gametangium in which the oospore forms after fertilization by the antheridium
were not produced in paired cultures using several known tester strains.

Asexual phase

SporangiaSporangia:
sac within which zoospores form, especially when water is cooled to about 10°C below ambient temperature; in solid substrates, sporangia usually germinate by germ tubes
were nonpapillatenonpapillate:
pertaining to the production of a non-distinct, or inconspicuous, papilla at the distal end of the sporangium (cf. papillate and semipapillate)
, persistentpersistent:
pertaining to sporangia that remain attached to the sporangiophore and do not separate or detach easily (cf. caducous)
, and predominantly ovoidovoid:
egg-shaped, with the widest part at the base of the sporangium and the narrow part at the apex
and obpyriformobpyriform:
inversely pear-shaped, i.e. with the widest part at the point of attachment (cf. pyriform)
in shape. SporangiaSporangia:
sac within which zoospores form, especially when water is cooled to about 10°C below ambient temperature; in solid substrates, sporangia usually germinate by germ tubes
averaged 35.9 x 26.2 µm (overall range 26.3–58.2 x 18.7–36.3 µm), proliferationproliferation:
formation of a sporangium within an empty sporangium that has previously emitted zoospores (internal or nested) or after the sporangiophore has emerged from the empty sporangium (external)
internal, nested, and extended. Sporangiophores unbranched. HyphaeHyphae:
single, tubular filament of a fungal or oomycete thallus; the basic structural unit of a fungus or oomycete
coralloid; hyphal swellings present. ChlamydosporesChlamydospores:
an asexual spore with a thickened inner wall that is delimited from the mycelium by a septum; may be terminal or intercalary, and survives for long periods in soil
absent.

Sexual phase

Sterile in culture.

Most typical characters

Resides in phylogenetic cladeclade:
a taxonomic group of organisms classified together on the basis of homologous features traced to a common ancestor
10b and is closely related to P. gallica and P. subarctica. Compared to these species, P. ukrainensis has small sporangiasporangia:
sac within which zoospores form, especially when water is cooled to about 10°C below ambient temperature; in solid substrates, sporangia usually germinate by germ tubes
and corraloid hyphaehyphae:
single, tubular filament of a fungal or oomycete thallus; the basic structural unit of a fungus or oomycete
.

Additional specimen(s) evaluated

Sweden, Kiruna area, isolated from forest stream, Sept. 2017, I. Milenković & T. Corcobado, SW154. Ukraine, Lviv region, Ivano-Frankove, isolated from naturally fallen Quercus leaves in the Vereshchytsia River, Aug. 2019, I. Milenković, T. Corcobado & I. Matsiakh, UA376, UA430, UA431, UA432, UA433.

Hosts and distribution

Distribution: Ukraine, Sweden
Substrate: forest stream
Disease note: no known disease
Host: no known host

Additional references and links

• SMML USDA-ARS: Phytophthora ukrainensis
• EPPO Global Database: Phytophthora ukrainensis
• Forest Phytophthoras of the world: Phytophthora ukrainensis
• CABI Digital Library: Phytophthora ukrainensis
• Encyclopedia of Life (EOL): Phytophthora ukrainensis
• Index Fungorum (IF): Phytophthora ukrainensis

Fact sheet author

Treena Burgess, Ph.D., Phytophthora Science and Management, Harry Butler Institute, Murdoch University, Australia

Z. Gloria Abad, Ph.D., USDA-APHIS-PPQ-S&T Plant Pathogen Confirmatory Diagnostics Laboratory (PPCDL), United States of America.